JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ353953.1
Database:	nt	Go to original record
Description:	Anthoxanthum monticola subsp. alpinum voucher Gillespie & Consaul 6859 CAN tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Anthoxanthum monticola subsp. alpinum
Original size:	1841 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	824	1434	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaac tccttctaaa
251   tttgagaaag aagggcttta tacatctaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacgcatat tataatatag gttctttatt
351   cttttttaga atgaaataga aatgattatg aaatcaaaaa ttctgaattt
401   ttttagaatt attgtgaatc cattccaatc gaatattgag taatcaaatc
451   cttcaattca aagttttgag atctttaaaa aagtggatta atcggacgag
501   gacaaagaga gagtcccatt ctacatgtca atactgacaa caatgaaatt
551   tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details