JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709444.1
Database:	nt	Go to original record
Description:	Eragrostis superba voucher KL59 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Eragrostis superba
Original size:	617 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	617	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaagcaagg ggttctcaaa
151   ctagaaccca aatgaaaagg ataggtgcag agactcaatg gaagctattc
201   taacgaatcg aggtgtaatt acgttgtgtt ggtagtgaaa gtccctctaa
251   atttgagaaa gaagggcttt atctaataca cacgtataga tactgacata
301   gcaaacgatt aatcacagaa cccctatcat aatataggtt ctttatttat
351   tttttagaat gaaattagga atgattatga aatagaaaat tctgaatttt
401   tattagaatt attgtgaatc cattccactc gaatattgag taatcaaatc
451   cttcaattca ttgtttttga gatcctttat ttttaaaaga ggattaatcg
501   tacgaggata aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details