JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709441.1
Database:	nt	Go to original record
Description:	Paspalum setaceum voucher KL56 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Paspalum setaceum
Original size:	616 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	616	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    cctgctaagt ggtaacttcc aaattcagag aaaccctgga atgaaaaatg
101   ggcaatcctg agccaaatcc cttttttgaa aaacaagtgg ttctcaaact
151   agaacccaaa agaaaaggat aggtgcagag actcaatgga agctgttcta
201   acgaatcgaa gtaattgcgt tgtgttggta gtggaacctc ttcgaaatta
251   gagaaagaag ggctttatac atctaatata cacgcatata tactgacata
301   gcaaacgatt aatcacggaa cccatatcat aatataggtt ccttatttta
351   ttttttgaat gaaattagga atgattatga aatagaaaat tctgaatttt
401   tttagaatta ttgtgaatcc attccaatcg aatattgagt aatcaaatcc
451   ttcaattcat tattttgttt tcgagatctt aaaaaaagtg gattaatcgg
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details