JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709431.1
Database:	nt	Go to original record
Description:	Croton texensis voucher KL46 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Croton texensis
Original size:	616 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	616	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgacaacttt caaattcagt gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcca aaaacaaata aaggttcaaa
151   aagacagaat aataataaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggggttgact gcgttgcatt agtaaagtaa agaaatcttt
251   ccgttaaaac tccagaaagg ataaagtaaa ggataaccct ataattcata
301   cgtattcgta ttgaaatact atctcatcaa ataattaatt attaattaat
351   aggtaatagg gcccgaatca atattttttt atctttctat gaaaaaaaat
401   atatatattg tttttgagtc gattccaagt tcaagaacgg attgaatttg
451   aattgatcaa atcattcact ccatagtctg atagataact gattaatcgg
501   acaagaataa agatagagtc ccgttctaca tgtcaatatc gacaacaagg
551   aaatttatag taagaggaaa atccgtcgac tttaaagatc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details