JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709412.1
Database:	nt	Go to original record
Description:	Rhynchosia senna voucher KL26 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Rhynchosia senna
Original size:	593 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	593	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagtct tggtatggaa
51    acttaccaag tgataacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgttttccg aaactaaata aaagttcaga
151   aagttataat aaaaaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaatggag ttgacgactt tttcttttgc agtaggaaag gaatctttcc
251   atccaaattc caggaatgga gcaaagagaa acataaagaa atatatatat
301   gtttctcttt aatgtactta actactattt caattgattc catttgtgat
351   aaaatttttc acaaatgaaa gatgtgaatt aaatcaattc caagttgaag
401   aaaagatgga atattcattg atcaaatcat tcactccatc ataatctgat
451   agatcctttg aagaactgat caatcagacg agaataaaga tagagtccta
501   ttctacatgt caatactgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgatttt ataaattgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details