JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709411.1
Database:	nt	Go to original record
Description:	Verbena halei voucher KL25 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Verbena halei
Original size:	548 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	548	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgacaaag agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgtgggt agaggaatct ttccatcgaa acttccgaaa
251   ggatgaagga taaacgtatc tattgaatac tctatcaaat gattaatgag
301   gtgtatctgt attttttgat atgaaaaatg gaagaattgg tgtgaattga
351   ttccacattg aagaaagaat cgaatattcc ttgatcaaat cattcactcc
401   atagtccgtc tttgaaagaa ttgattaatc ggacgagaat aaagatagag
451   tcccattcta catgtcaata ccggcaagaa tgaaatttat tgtaagagga
501   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details