JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709407.1
Database:	nt	Go to original record
Description:	Setaria scheelei voucher KL21 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Setaria scheelei
Original size:	615 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	615	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaaa
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctcgaaat
251   actagaaaga aggaacttta tacatttaat acacacgtat agatactgac
301   atagcaaacg attaatcaca gaacccctat cataatatag gttctttatt
351   ttatattttt tttttagaat gaaattagga atgattatga aatagaaaat
401   tctgaatttt tttagaatta ttgtgaatcc attccaatcg aatattgagt
451   aatcaaatcc ttcaattcat tgttttgaga tcaaaagtgg attaatcgaa
501   cgaggataaa gagagagtcc cattctacat gtcaatactg acaacaatga
551   aatttctagt aaaaggaaaa tccgtcgact ttataagtcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details