JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709402.1
Database:	nt	Go to original record
Description:	Yucca constricta voucher KL15 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Yucca constricta
Original size:	574 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	574	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttattttta gaaaaacaag ggtttagact
151   agaataaaaa gggataggtg cagagactca atggaagctg ttctaacgaa
201   tggagttgac tacgttgcgt tgataactgg aattcttcta tcgaaattaa
251   agaaaggatg acgtatatat ctaatacgta cgtatacata ctggcatatc
301   aaacgattaa tcacgacttg aatatatata tatgcaatat atgcaaaatt
351   cagagtggat ctattccaat cgaagttgaa ggaagaatct aatattcagt
401   gatcaaatca ttcattccag agtttgatag accttttttt gaaaaactga
451   ttaattggac gagaataaag agagagtccc attctacatg tcaataccga
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt tagaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details