JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709397.1
Database:	nt	Go to original record
Description:	Nothoscordum bivalve voucher KL08 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Nothoscordum bivalve
Original size:	576 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	576	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttctttttt gaaaaacaaa gggttttaaa
151   aactagaata aaaagggata ggtgcagaga ctcaatggaa gctgttctaa
201   cgaatggagt tgactacgtt gcgttggtaa ctggaatcct tctatcgaaa
251   ttcaagaaag gatgacttat atatctaata catacgtata catactggca
301   tatcaaacga ttaatcacga cccaaatcca ttatatatat atgcaaaatt
351   cagagttatt gtggatctat tccattcgaa gttgaaggaa gaatcgaata
401   tttagtgatc aaatcattca ttccagagtt tgatagacct tttttttaat
451   gattaatcgg acgagaataa agagagagtc ccgttctaca tgtcaataac
501   gacaacaatg aaatttatag taagaggaaa atccgtcgac tttagaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details