JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709454.1
Database:	nt	Go to original record
Description:	Hopia obtusa voucher KL71 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Hopia obtusa
Original size:	616 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	616	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aagaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaattgcg ttgtgttggt agtggaacct cttcgaaatt
251   agagaaagaa gggctttata catctaatat acacgcatat atactgacat
301   agcaaacgat taatcacgga acccatatca taatatggtt ccttatttta
351   ttttttgaat gaaattagga atgattatga aatagaaaat tctgaatttt
401   tttagaatta ttgtgaatcc attccaatcg aatattgagt aatcaaatcc
451   ttcaattcat tattttgttt tcgagatctt aaaaaaagtg gattaatcag
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details