JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709394.1
Database:	nt	Go to original record
Description:	Disakisperma dubium voucher KL05 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Disakisperma dubium
Original size:	618 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	618	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtgtaatta cgttgtgttg gtagtgaaac tccctctaaa
251   ttactaaatt agagaaagaa gggctttata catctaatac acacgtatag
301   atactgacat agcaaacgat taatcacaga acccataata taggttcttt
351   atttattttt tagaatgaaa ttaggaatga ttatgaaata gaaaattctg
401   attttttttt tagaattatt gtgaatctat tccactcgaa tattgagtaa
451   tcaaatcctt caattcattg tttttgagat cttttaaaaa tagattaatc
501   ggacgaggat aaagagagag tcccattcta catgtcaata ctgacaacaa
551   tgaaatttct agtaaaagga aaatccgtcg actttataag tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details