JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709388.1
Database:	nt	Go to original record
Description:	Quercus marilandica voucher AH07 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Quercus marilandica
Original size:	559 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	559	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctattttacg aaaaacaaat aagggttcag
151   aagaaagcaa gaataaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaag tggggttgac ttctttacgt tattacatta acgtaatcct
251   tatatcaaaa ctacagaaag gataaaccta tatacatacg tatatgtact
301   gaaatcctat ctcaaatgat taatgacgac ccgaatcttt atttatttat
351   atttctataa ataataaata aaaatcgaaa gagttgttgt gaatcgatcc
401   aaattgaaga aagaatcgaa tatttattaa taaaatttat cgtacgagaa
451   taaagataga gtcccattcc acacgtcaat accgacaaga atgaaattta
501   taggaagagg aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details