JRC GMO-Amplicons | EURL GMFF

Record ID:	MG709382.1
Database:	nt	Go to original record
Description:	Sideroxylon lanuginosum voucher AH01 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Sideroxylon lanuginosum
Original size:	583 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	583	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cgaaaataaa caaagattca
151   gaaagcgaaa ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg cgttggtaaa agaatccttc catcaaaact
251   tcagaaagga tgaaagataa acctatatac atacgcatac atactgaaat
301   cctatatcaa ataattaatg acgacccaaa tctgtatccg tcttttttta
351   tatgaaaatg gaagaattgt tgcgaatgga ttccacattg aataaagaat
401   tgaatattca ttgatcaaat catttactcc atagtttgat agatcttttg
451   aagaactgat taatcggacg agaataaaga tagagtccca ttctacatgt
501   caataccgac aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
551   agaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details