JRC GMO-Amplicons | EURL GMFF

Record ID:	LN877396.1
Database:	nt	Go to original record
Description:	Dianthus moravicus chloroplast genome, complete sequence, isolate dm3637
Taxon:	Dianthus moravicus
Original size:	149524 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46504	47122	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aaaaaaaaaa
101   aggggtaatc ctgagccaac tccttttgtt tccttttttc aaaagaaaaa
151   aaggataggt gcagagactc aaaggaagct gttctaacaa attgggttga
201   ctgtgctgtg ttgttataag acttcttccg tctaaattcc cataaaaaag
251   ggggggtaaa atcgacggag tgaaatgatt aatgacaacc cgaatctgtt
301   ctgtattttt ttttatatat tatatatata aatccatact ttattctata
351   tttagagtag agatctagaa gattaaatga aaaaatgcaa gaattcttgt
401   gaatccattc aaagttgaaa gcagaatcaa tcaagattta ttcattaaaa
451   tattcacact aactccatag tctgatagat attgtgaaga acttattaat
501   cggatgagaa taaagataga gtcccgtact acatgtcaat actgacaaca
551   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcatgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details