JRC GMO-Amplicons | EURL GMFF

Record ID:	LN877395.1
Database:	nt	Go to original record
Description:	Dianthus gratianopolitanus chloroplast genome, complete sequence, isolate dg3051
Taxon:	Dianthus gratianopolitanus
Original size:	149670 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46629	47246	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   agggtaatcc tgagccaact ccttttgttt ccttttttca aaagaaaaaa
151   aggataggtg cagagactca aaggaagctg ttctaacaaa ttgggttgac
201   tgtgctgtgt tgttataaga cttcttccgt ctaaattccc ataaaaaagg
251   gggggtaaaa tcgacggagt gaaatgatta atgacaaccc gaatctgttc
301   tgtatttttt tttatatatt atatatataa atccatactt tattctatat
351   ttagagtaga gatctagaag attaaatgaa aaaatgcaag aattcttgtg
401   aatccattca aagttgaaag cagaatcaat caagatttat tcattaaaat
451   attcacacta actccatagt ctgatagata ttgtgaagaa cttattaatc
501   ggatgagaat aaagatagag tcccgtacta catgtcaata ctgacaacaa
551   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcatgagggt
601   tcaagtccct ctatcccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details