JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926180.1
Database:	nt	Go to original record
Description:	Myrsine seguinii voucher Wang2007244 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Myrsine seguinii
Original size:	903 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	575	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgaaccaaa tcctcttttt cgaaaacaaa gattaaagga
151   aaataaaaaa gagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg atcgcgttgg tagaggaatc ttttcatcaa aacttcagaa
251   aggatgaaag ataaacgtat atacatatgc atatgcatat gtactgaaat
301   cctatatcaa aataaaatgc ttattttttt ctatgaaaaa tagaagaatc
351   gttgcgaatc gattccacat tccacattga ataaagaatt tcatattcat
401   tgatcaaatc atttactcca tagtctaata gatcttgtga ataactgatt
451   aatcaggcaa gaataaagat agagtcccat tctacatgtc aataccgaca
501   acaataatga aatttatagt acgaggaaaa tccgtcgact ttataaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details