JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926179.1
Database:	nt	Go to original record
Description:	Myrsine semiserrata voucher Wang2007230 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Myrsine semiserrata
Original size:	930 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	589	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgaaccaaa tcctcttttt cgaaaacaaa gattaaattc
151   gaaaacaaag attaaaggaa aataaaaaag agggataggt gcagagactc
201   aatggaagct gttctaacaa atggagttga tcgcgttggt agaggaatct
251   tttcatcaaa acttcagaac ggatgaaaga taaacgtata tatatatgca
301   tatgtactga aatcctatat caaaataaga tgcttatttt ttttctatga
351   aaaatagaag aatcgttgcg aatcgattcc acattccaca ttgaataaag
401   aatttcatat tcattgatca aatcatttac tccatagtct aatagatctt
451   gtgaataact gattaatcag gcaagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaacaata atgaaattta tagtacgagg aaaatccgtc
551   gactttataa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details