JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926178.1
Database:	nt	Go to original record
Description:	Maesa japonica voucher Wang2007196 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Maesa japonica
Original size:	895 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	569	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaaa tcctgttttt caaaaacaaa caaagattaa
151   aaaagtgaaa ataaaaaaag gggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaatcct cccatcatcc
251   ttcagaaagg atgaaaaaga aacctatata catatgcata cgtactgaaa
301   tactatatca aaaaaaatgc ttattttttt tttgatatga aaaatggaag
351   aatctttgaa aatcgattcc acattgaata aagaatttca tattcattga
401   tgaaatcatt tactccatag cctgatagat cttgtgaata actgattaat
451   cgggcaagaa taaagataga gtcccattct acatgtcaat accgacaaca
501   atgaaattta tagtaaaagg aaaatccgcc aactttagaa atccggaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details