JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926177.1
Database:	nt	Go to original record
Description:	Embelia ribes subsp. pachyphylla voucher Wang2007250 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Embelia ribes subsp. pachyphylla
Original size:	911 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaaa tcctcttttt tgaaaacaaa gattaaagga
151   aaataaaaaa gagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg atcgcattgg tagaggaatc ttttcatcaa aacatcagaa
251   aggatgaaag ataaacgtat atacatatgc atatgtactg aaatcctata
301   tcaaaataaa atcaaaataa aatgcttctt tttttttctt tctataaaaa
351   atagaagaat cgttgcgaat cgattccaca ttccacattg aataaaaaat
401   ttcctattca ttgatcaaat catttactcc atagtctaat agatcttgtg
451   aataactgat tcatcaggca agaataaaga tagagtccca ttctacatgt
501   caataccgac aacaataatg aaatttatag tacgaggaaa atccgtcgac
551   tttagaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details