JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926176.1
Database:	nt	Go to original record
Description:	Aegiceras corniculatum voucher Zheng200901 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Aegiceras corniculatum
Original size:	888 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	567	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   acgggcaatc ctgaaccaaa tcctcttttt cgaaaacaaa gattaaagga
151   aaataaaaaa gagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg atcgcgttgg tagaggaatc ttttcatcaa aacttcagaa
251   aggatgaaag atgtatatac atatgcatat gtactgaaat cctatatcaa
301   aatagaatgc ttattttttt ttctatgaaa agtagaagaa tcgttgcgaa
351   tcgattccac attccacatt gaataaagaa tttcatattt attgatcaaa
401   tcatttactc catagtctaa tagatcttgt gaataactga ttaatcaggc
451   aagaataaag atagagtccc attctacatg tcaataccga caacaataat
501   gaaatttata gtacgaggaa aatccgtcga ctttataaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details