JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926157.1
Database:	nt	Go to original record
Description:	Ardisia pubivenula voucher Wang200795 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ardisia pubivenula
Original size:	899 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	570	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaaa tcctcttttt cgaaaacaaa gattaaagga
151   aaataaaaaa gagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg atcgcgttgg tagaggaatc ttttcatcaa aacttcagaa
251   aggatgaaag ataaacgtat atacatatgc atatgtactg aaaccctata
301   tcaaaataaa atgcttattt tttctttcta tgaaaaatag aagaatcgtt
351   gcgaatcgat tccacattcc acattgaata aaaaatttca tattcattga
401   tcaaatcatt tactccatag tctaatagat cttgaataac tgattaatca
451   ggcaagaata aagatagagt cccattctac atgtcaatac cgacaacaat
501   aatgaaattt atagtacgag gaaaatccgt cgactttata aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details