JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926147.1
Database:	nt	Go to original record
Description:	Ardisia obtusa voucher Wang2007302 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ardisia obtusa
Original size:	911 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	579	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaaa tcctcttttt cgaaaacaaa gattaaagga
151   aaataaaaaa gagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg atcgcgttgg tagaggaatc ttttcatcaa aacttcagaa
251   aggatgaaag ataaacgtat atacatatgc atatgtactg aaatcctata
301   tcaaaataaa atgcttattt tttctttttt ctttctatga aaaatagaag
351   aatcgttgcg aatcgattcc acattccaca ttgaataaaa aatttcatat
401   tcattgatca aatcatttac tccatagtct aatagatctt gtgaataact
451   gattaatcag gcaagaataa agatagagtc ccattctaca tgtcaatacc
501   gacaacaata atgaaattta tagtacgagg aaaatccgtc gactttataa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details