JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926136.1
Database:	nt	Go to original record
Description:	Ardisia fordii voucher Wang200845 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ardisia fordii
Original size:	895 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	565	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaaa tcctcttttt cgaaaacaaa gattaaagga
151   aaataaaaaa gagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg atcgcgttgg tagaggaatc ttttcatcaa aacttcagaa
251   aggatgaaag ataaacgtat atacatatgc atatgtactg aaatcctata
301   tcaaaataaa atgcttattt tttctttcta tgaaaaatag aagaatcgtt
351   gcgaatcgat tccacattga ataaaaaatt tcatattcat tgatcaaatc
401   atttactcca tagtctaata gatcttgtga ataactgatt aatcaggcaa
451   gaataaagat agagtcccat tctacatgtc aatatcgaca acaataatga
501   aatttatagt acgaggaaaa tccgtcgact ttataaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details