JRC GMO-Amplicons | EURL GMFF

Record ID:	EU220739.1
Database:	nt	Go to original record
Description:	Salvia miltiorrhiza f. alba from China tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia miltiorrhiza f. alba
Original size:	883 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	524	+	524 bp

Amplicon:

524 bp

1     cgaaatcggt agacgctacg gacttattag atgagcttgg tatggaaact
51    tactaagtga tacttcaaat tcagagaaac cccggaatta ataaaaatgg
101   gcaatcctga gccaaatcct gttttttcaa aaaaaaggat aggtgcagag
151   actcaatgga agttgttcta acaaatggag ttgactgcgc cggtagagga
201   atctttccac ggaaacttta gaaaggatga aggataaacc catctattga
251   atactatatc aaatgattaa tgttggcccg aatctgtttt tttaatatga
301   aaatggaaaa atcgatgtga atttatttca cgttgaagaa taaatcgaat
351   attcatcaac tcattcactc catagttcga tagatctttt aaagaactta
401   ttaatcggac gagaataaag atagagtccc attctacatg tcaataccgg
451   caacaatgaa atttatagta agaggaaaat ccgtcgactt taaaaatcgt
501   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.36e-08
primerB	100.0	20 bp	0	0	524	505	2.36e-08

Match details