JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926126.1
Database:	nt	Go to original record
Description:	Ardisia conspersa voucher Wang2007260 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ardisia conspersa
Original size:	911 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	582	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaaa tcctcttttt cgaaaacaaa gattaaagga
151   aaataaaaaa gagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg atcgcgttgg tagaggaatc ttttcatcaa aacttcagaa
251   aggatgaaag ataaacgtat ataaacgtat atacatatgc atatgtactg
301   aaatcctata tcaaaataaa atgcttattt tttctttcta tgaaaaatag
351   aagaatcgtt gcgaatcgat tccacattcc acattgaata aaaaatttca
401   tattcattga tcaaatcatt tactccatag tctaatagat cttgtgaata
451   actgattaat caggcaagaa taaagataga gtcccattct acatgtcaat
501   accgacaaca ataatgaaat ttatagtacg aggaaaatcc gtcgacttta
551   taaatcgtga gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details