JRC GMO-Amplicons | EURL GMFF

Record ID:	MF926118.1
Database:	nt	Go to original record
Description:	Ardisia alyxiifolia voucher Wang2007122 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Ardisia alyxiifolia
Original size:	912 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	583	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    cctactaagt gagaactttc aaattcagag aaaccctgga attaataaaa
101   atgggcaatc ctgagccaaa tcctcttttt cgaaaacaaa gattaaagga
151   aaataaaaaa gggggatagg tgcagagact caacggaagc tgttctaaca
201   aatggagttg atcgcgttgg tagaggaatc ttttcatcaa aacttcagaa
251   aggatgaaag ataaacgtat tataaacgta tatacatatg catatgtact
301   gaaatcctat atcaaaataa aatgcttatt ttttctttct atgaaaaata
351   gaagaatcgt tgcgaatcga ttccacattc cacattgaat aaaaaatttc
401   atattcattg atcaaatcat ttactccata gtctaataga tcttgtgaat
451   aactgattaa tcaggcaaga ataaagatag agtcccattc tacatgtcaa
501   taccgacaac aataatgaaa tttatagtac gaggaaaatc cgtcgacttt
551   ataaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details