JRC GMO-Amplicons | EURL GMFF

Record ID:	MH256801.1
Database:	nt	Go to original record
Description:	Gomphogyne cissiformis var. cissiformis chloroplast, complete genome.
Taxon:	Gomphogyne cissiformis var. cissiformis
Original size:	157334 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	50246	50853	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttcaga aaacaaataa gattaaaggc
151   ttcgaaaggg agagcgagaa aaaaagggta ggtacagaga ctcaatggaa
201   gctgttctaa caaatggagt tgactacgtt gcgttagtaa aggaatgaat
251   ccttccatcg aaactccaga aaagatgaaa gataaacata tatacgcact
301   gaaatactat ttgattaatg acgacccgaa tctctctttt tttttagtta
351   tctaaaactg ataaaaaaaa cgaattggta ttaatcgatt tcaatagctc
401   cgagttgaaa aaagaatcga atattcattc atcaaatcag ttactccatc
451   atagtctgat agatctttta aagaacggat taatcagatg agaatagaat
501   aaagatagag tcccattcta catgtcaata ccgacaaaaa tgaaatttat
551   agtaagagga aaatccgtcg acttaaaaaa tcgtgagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details