JRC GMO-Amplicons | EURL GMFF

Record ID:	MH394400.1
Database:	nt	Go to original record
Description:	Meconopsis racemosa chloroplast, complete genome.
Taxon:	Meconopsis racemosa
Original size:	153729 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46667	47270	+	604 bp

Amplicon:

604 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcat aaaacaaatt cagggatttc
151   tgaaagcgag aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaatgaat ggagttgact gcgttggtag aggaatcctt cttttaaatt
251   ttaatttaaa ctccataaag aatgagggat gaacctatat acgatacata
301   cgtatacgta ctgaaatatc aaagattcta ttaatgccgc cctaaatata
351   ttatattttt tttacataaa aaaaggaata atttttgtga atctattcca
401   agttgaagga agaattgaaa attcagtgat caaaccattc actccatagt
451   ctgatagatc ttttgacgaa ttgattaatc ggacgagaat aaagatagag
501   tcccgttcta catgtcaata tcaataccga caacaatgaa atttatagta
551   agaggaaaat ccgtcgactt tataaatcgt gagggttcaa gtccctctat
601   cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.72e-08
primerB	100.0	20 bp	0	0	604	585	2.72e-08

Match details