JRC GMO-Amplicons | EURL GMFF

Record ID:	EU220730.1
Database:	nt	Go to original record
Description:	Salvia tricuspis from China tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia tricuspis
Original size:	890 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	530	+	530 bp

Amplicon:

530 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgatgacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa aaggataggt
151   gcagagactc gatggaagtt gttctaacaa atggagttga ctgcgccggt
201   agaggaatct ttccacggaa actttagaaa ggatgaagga taaacccatc
251   tattgaatac tatatcaaat gattaatgtt ggcccgaatc tgttttttta
301   atatgaaaat ggaaaaatcg atgtgaattt atttcacgtt gaagaataaa
351   tcgaatattc atcaactcat tcactccata gttcgataga tcttttaaag
401   aacttattaa tcggacgaga ataaagatag agtcccattc tacatgtcaa
451   taccggcaac aatgaaattt atagtaagag gaaaatccgt cgactttaaa
501   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	530	511	2.38e-08

Match details