JRC GMO-Amplicons | EURL GMFF

Record ID:	MH019242.1
Database:	nt	Go to original record
Description:	Physalis peruviana chloroplast, complete genome.
Taxon:	Physalis peruviana
Original size:	156706 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49125	49700	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagctatt ctaacaaatg
201   gagttaaatg cgttggtaga ggaatcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatata catacgtatt gaatactata
301   tcaaatgatt aatgacgacc cgaatccgta ttttttctat aaaaaataga
351   agaattggtg tgaatcgatt ctacattgaa gaaagaatcg aatattcatt
401   gatcaaatca ttcaattcac tccatagtct gatagatctt ttgaagaact
451   gattaatcgg acgagaataa agatagagtc ccgttctaca tgtcaatgcc
501   ggcaacaatg aaatttatag taagaggaaa atccgtcgac tttaaaaatc
551   gtgagggttc aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details