JRC GMO-Amplicons | EURL GMFF

Record ID:	MH150979.1
Database:	nt	Go to original record
Description:	Oryza sativa cultivar Bayar Putih trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Oryza sativa
Original size:	946 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	321	936	-	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tatatgagcc ttggtatgga
51    aacctgctaa gtggcaactt ccaaattcag agaaaccctg gaattaaaaa
101   aggggcaatc ctgagccaaa tccatgtttt gagaaaacaa gcggttctcg
151   aactagaacc caaaggaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacgaat cgagttaatt acgttgtgtt gctagcggaa ctcccttcta
251   aattagggaa agaagggctt tcgaaatcta atacacacgt atagatactg
301   gcatagcaaa cgattaatca cagaactcat atcataatat aggttcttta
351   attctttttt aaaatgaaaa taggaatgat tatgaaatag aaaattcata
401   atttttttag aattgttgtg aatccattcc aatcgaatat tgagtaatca
451   aatccttcaa ttcatagttt tcgaaatctt tttaaaagcg gattaatcgg
501   acgaggataa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttctaagtc gtgagggttc
601   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details