JRC GMO-Amplicons | EURL GMFF

Record ID:	EU200173.1
Database:	nt	Go to original record
Description:	Salvia flava tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia flava
Original size:	889 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	529	+	529 bp

Amplicon:

529 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgaaaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaaaaa aggataggtg
151   cagagactca atggaagttg ttctaacaaa tggagttgac tgcgccggta
201   gaggaatctt tccacggaaa ctttcgaaag gatgaaggat aaacccatct
251   attgaatact atatcaaatg attaatgttg gcccgaatct gtttttttaa
301   tatgaaaatg gaaaaatcga tgtgaattta tttcacgttg aagaataaat
351   caaatattca tcaactcatt cactccatag ttcgatagat cttttaaaga
401   acttattaat cggacgagaa taaagataga gtcccattct acatgtcaat
451   accggcaaca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
501   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	529	510	2.38e-08

Match details