JRC GMO-Amplicons | EURL GMFF

Record ID:	EU200170.1
Database:	nt	Go to original record
Description:	Salvia cavaleriei var. simplicifolia tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Salvia cavaleriei var. simplicifolia
Original size:	889 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	530	+	530 bp

Amplicon:

530 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tttcaaaaaa aaaggatagg
151   tgcagagact caatggaagt tgttctaaca aatggagttg actgcgccgg
201   tagaggaatc tttccacgga aactttagaa aggatgaagg ataaacccat
251   ctattgaata ctatatcaaa tgattaatgt tggcccgaat ctgtttttta
301   atatgaaaat ggaaaaatcg atgtgaattt ctttcacgtt gaagaataaa
351   tcgaatattc atcaactcat tcactccata gttcgataga tcttttaaag
401   aacttattaa tcggacgaga ataaagatag agtcccattc tacatgtcaa
451   taccggcaac aatgaaattt atagtaagag gaaaatccgt cgactttaaa
501   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.38e-08
primerB	100.0	20 bp	0	0	530	511	2.38e-08

Match details