JRC GMO-Amplicons | EURL GMFF

Record ID:	EU191023.1
Database:	nt	Go to original record
Description:	Ficus carica cultivar Sawoudi tRNA-Leu (trnL) gene, intron; chloroplast.
Taxon:	Ficus carica
Original size:	588 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	584	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaatca tttatgattg agcgttggta
51    tggaaaccta ccaagtgata actttcaaat tcagagaaac cctggaatta
101   aaaatgggca atcctgagcc aaatccggtt ttctgaaaac aaacaagggt
151   tcagaaggcg ataataaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttggc tgcggtgcgt tagtaaagga atcactccag
251   aaaggatgaa gaataaacct atatacgtat acgtactgaa atagtatctt
301   caaatgatta atgacaaccc aaatccgtat ttcttttaat tttcatgaaa
351   aattaaagaa ttattgtaaa tcaattatta agttgaaaaa agaattaaat
401   attcattaat caaatcattt actccatcaa aatctgatag atcttttgaa
451   gaatggatta atcggacgag aataaagata gagtcccatt tttcatgtca
501   atattcgaca acaatgaaat ttatagtaag aggaaaatac cgtcgacttt
551   aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details