JRC GMO-Amplicons | EURL GMFF

Record ID:	LS453403.1
Database:	nt	Go to original record
Description:	Iresine latifolia trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence, isolate AC0801
Taxon:	Iresine latifolia
Original size:	1027 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	106	652	+	547 bp

Amplicon:

547 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aaaatgggca atcctgagcc aactccttta ttaggaaaaa aaaaaataag
151   aattcagaaa acaagaataa aaaaaaaaca aaggataggt gcagagactc
201   gaaggaagct gttctaacaa atggagttga ctgtgctgtg ttgttctaag
251   aattctactt caatccaata tgaaatgaaa atatatatat aattattatt
301   atgaatatga aaaaatggaa gaatttttac gaatcaaatt caagttgaaa
351   tttgaaaaaa gaatcaaata ttcattcatc aaatcattca ctccatagtc
401   tgatagatct tttgaagagc tgattaatcg gacgagaata aagatagagt
451   cccattctac atgtcaatac tgacaacaat gaaatttata gtaagaggaa
501   aatccgtcga ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	547	528	2.46e-08

Match details