JRC GMO-Amplicons | EURL GMFF

Record ID:	LS453401.1
Database:	nt	Go to original record
Description:	Iresine pringlei trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence, isolate AC0799
Taxon:	Iresine pringlei
Original size:	1049 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	109	656	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aaaatgggca atcctgagcc aactccttta ttaggaaaaa aaataagaat
151   tcagaaaaca agaataaaaa aaaacaaagg ataggtgcag agactcaaag
201   gaagctgttc taacaaatgg agttgactgt gctgtgttat tctaagaatt
251   ctacttcaat ccaatatgaa atgaaaaaaa actatatata taattattat
301   tatgaatatg aaaaaataga agaattttta cgaatcaaat tcaagttgaa
351   atttgaaaaa agaatcaaat attcattcat caaatcattc actccatagt
401   ctgatagatc ctttgaagag ctgattaatc ggacgagaac aaagatagag
451   tcccattcta catgtcaata ctgacaacaa tgaaatttat agtaagagga
501   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details