JRC GMO-Amplicons | EURL GMFF

Record ID:	MG573056.1
Database:	nt	Go to original record
Description:	Tiquilia plicata voucher TRTE:Stefanovic-16-23 chloroplast, complete genome.
Taxon:	Tiquilia plicata
Original size:	154559 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48173	48754	+	582 bp

Amplicon:

582 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa aaaaggttca
151   gaaagcgaaa aaaaggatag gtgcagagac tcgatggaag ctgttctaac
201   gaatggagtt gactgccttg gtcgaggaat ctttccatcg aaacttccga
251   aaggatggag gataaacgta tatacatatg tattcaatac tctatcaaat
301   gaaatgatta atgatgaccc taaacattct ggtttttttt atgttatgaa
351   aaaaaaaaga aagagttggt ctgagttgat tccacattga agaaagggtc
401   gaatattcat tgatcaaatc attcactcca taatctgata gatcttttga
451   agaactgatt aatcggacga gaataaagat agagtcccgt tctacatgtc
501   aataccggca aaaatgaaat ttatagtaag aggaaaatcc gtcgatttaa
551   aacatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	582	563	2.62e-08

Match details