JRC GMO-Amplicons | EURL GMFF

Record ID:	MH011354.1
Database:	nt	Go to original record
Description:	Notholirion macrophyllum voucher DYJ2017081601 chloroplast, complete genome.
Taxon:	Notholirion macrophyllum
Original size:	152143 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45543	46155	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgatta aattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccccgg aattaaaaat
101   gggcaatcct gagccaaatc tttattttga gaaaaagggt ttaatttata
151   gtttttataa tataaaacta caatcaaaaa aagataggtg cagagactca
201   atggaagctg ttctaacgaa tgaagttgat tacgttgcgc tggtagccgg
251   aatccttcta tcaaaattac ggagaggatg cccgccctat atacgtatat
301   atacatactg acatagtaaa cgattgatta atcgcggccc gaatccatta
351   taatatatat atatatatga aaaatttaag agttattgta aatctatatc
401   catattccaa tcaaagttta aggaagaatc gaatattcag tgatcaaatt
451   attcattcca gagtctgtat agatcttttt aaaaactgat tattcggacg
501   agaataaaga gagagtccca ttctacgtgt caataccgac aacaatgaaa
551   tttatagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details