JRC GMO-Amplicons | EURL GMFF

Record ID:	MF491390.1
Database:	nt	Go to original record
Description:	Fagopyrum dibotrys chloroplast, complete genome.
Taxon:	Fagopyrum dibotrys
Original size:	159919 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48328	48904	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaatta ggttgagcct tagtagggaa
51    acctactagg tgagaacttt caaattcaga gaaaccctgg aatcaaaaaa
101   cgggtaatcc tgagccaact cctgctttcc aaaagaaaga ataaatacag
151   gataggtgca gagactcaat ggaagctgtt ctaacaaaat ggagttgact
201   gtcttgcgtt gttataagaa ctcttccgtc gaaactcaaa aaaggatgaa
251   gaataaacgt atacgcatac atacgtcaat ataaaatact aaaaaaaatg
301   aaaaatattc ataacgaccc gactttttat tttatgacta tgaaaaaagg
351   aagaattgtt atgaatcgat ttcacgatga agaaagaatc gaatattctt
401   ttagtaaata aaagcattta cttccccgtc tgatagatct tttgaaaaac
451   cgatccatcg gatgagaatg aagatagagt cccattctac gtgtcaatcc
501   tgacaacaaa gaaatttata gtaagaggaa aatccgtcga ctttataaat
551   cgtgagggtt caagtccctc tatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details