JRC GMO-Amplicons | EURL GMFF

Record ID:	KU750660.1
Database:	nt	Go to original record
Description:	Synotis alata voucher R396 trnT-UGU-trnL-UAA intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene, trnL-UAA-trnF-GAA intergenic spacer, tRNA-Phe (trnF-GAA) gene, and trnF-GAA-ndhJ intergenic spacer, complete sequence; and ndhJ (ndhJ) gene, partial cds; chloroplast.
Taxon:	Synotis alata
Original size:	2293 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	573	1107	+	535 bp

Amplicon:

535 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aataaaaaag gataggtgca gagactcgat ggaagttgtt
201   ctaacgaatg gagttgattg tcttacattg gtagaggaat ccttctatcg
251   aaacttcaga aaagatgaag gataaaagaa aagatgaagg ataaacctgt
301   atacataata cagaagaatt ggtatgaatc gattccatat tgaagaaaga
351   atcgaatatc cattgatcaa aacattcact ccataatctg atagatcttt
401   tgaagaactg attaatcgga cgagaataaa gatagagtcc cgttctacat
451   gtcaataccg gcaacaatga aatttatagt aagaggaaaa tccgtcgatt
501   tcaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.41e-08
primerB	100.0	20 bp	0	0	535	516	2.41e-08

Match details