JRC GMO-Amplicons | EURL GMFF

Record ID:	KU750636.1
Database:	nt	Go to original record
Description:	Sinosenecio hupingshanensis voucher Y613 trnT-UGU-trnL-UAA intergenic spacer, partial sequence; tRNA-Leu (trnL-UAA) gene, trnL-UAA-trnF-GAA intergenic spacer, tRNA-Phe (trnF-GAA) gene, and trnF-GAA-ndhJ intergenic spacer, complete sequence; and ndhJ (ndhJ) gene, partial cds; chloroplast.
Taxon:	Sinosenecio hupingshanensis
Original size:	2398 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	625	1152	+	528 bp

Amplicon:

528 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaaacgaa aataaaaaag gataggtgca gagactcgat ggaagttgtt
201   ctaacgaatg gagttgattg tcttacattg gtagaggaat ccttctatcg
251   aaacttcaga aaagactatc gaaacttcag aaaagatgaa ggataaacct
301   gtatacacaa tacagaagaa ttggtatgaa tcaattccat attgaagaaa
351   gaatcgaata tccattgatc aaaccattca ctccataatc tgataaatct
401   ttgattaatc ggacgagaat aaagatagag tcccgttcta catgtcaata
451   ccggcaacaa tgaaatttat agtaagagga aaatccgtcg atttcaaaaa
501   tcatgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	528	509	2.37e-08

Match details