JRC GMO-Amplicons | EURL GMFF

Record ID:	LT996928.1
Database:	nt	Go to original record
Description:	Amaranthus sp. MW-2265 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence, isolate AC0940.
Taxon:	Amaranthus sp. MW-2265
Original size:	1096 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	117	729	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcctttttc aaaagtaaaa aaaaatacgg
151   attcagaaag caagaatcaa aaaaaggata ggtgcagaga ctcaaaggaa
201   gctgttctaa caaatggagt tgattgtgct gggttgttcg aagaattctc
251   cgtcgaaatt cctgattaat gacacttcga atctattctg tatttctatt
301   tttagaattt ttagatattt cttatttcta gataatatag aatatgaaat
351   tttctagaaa taaaaatgaa tatgaaaaaa tggaagaatt tttgtgactc
401   tatttcaagt tgaaaaaaga atcaaatatt catcaaatca ttcactccat
451   agtctgatag atctttcttt gaaagaaccg gttaatcggt tactcggacg
501   agaataaaga tagagtccca ttatacatgt caatactgac aacaatgaaa
551   tttatagtac gaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details