JRC GMO-Amplicons | EURL GMFF

Record ID:	LT996927.1
Database:	nt	Go to original record
Description:	Lagrezia sp. Kiefer 9498 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence, isolate AC0896.
Taxon:	Lagrezia sp. Kiefer 9498
Original size:	1068 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	108	707	+	600 bp

Amplicon:

600 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctgctaag tgaaaacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa ctcctttttt tttttcaaaa gtaaaaacaa
151   aagaaggatt cagaaagcaa gaataaaaaa caaaggatag gtgcagagac
201   tcaaaggaag ctgttctaat aaatggagtt gactgtgctg tgttgttctc
251   agaattcttt cttcgaaact attctgcatt tctatttttc gaatttttag
301   atatttctta tttctatata gcataaaata ggagattttc gataaataaa
351   aatttcgaat atgaatagga aaaaatggaa gaatttttgt caattaattt
401   caagttgaaa aaagaatcaa atattcgctc aacaaatcat tcactccata
451   gtctgataga tctttgcaag aacttattaa tcggacgaga ataaagatag
501   agtcccattc tacatgtcaa tactgacagc aatgaaattt atagtaaaag
551   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	600	581	2.70e-08

Match details