JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963272.1
Database:	nt	Go to original record
Description:	Tonduzia stenophylla chloroplast, partial genome.
Taxon:	Tonduzia stenophylla
Original size:	128886 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48194	48758	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaatcccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggccg cgttggtaga gaaatctttc catcgaaaat tcagaaagga
251   tgaaggataa acgtatatac atacgtattg aatactatat caaatgatta
301   atgccgaccc gaatgaatct gtattttttc tataaaaaaa atgtaagaat
351   tggtgtgatt cgattccaca ttgaagaaag aatcgaatat tcattgatca
401   aatgattcac tccatagtct gtaaatcttt tcaagaactg attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgctg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact taaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details