JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963271.1
Database:	nt	Go to original record
Description:	Skytanthus acutus chloroplast, partial genome.
Taxon:	Skytanthus acutus
Original size:	128408 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47724	48287	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaactcaga gaaaccccgg aattaataaa
101   aggggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagcaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggcc acgttggtag aaaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaggata aacgtatata catacgtatt gaatactata tcaaatgatt
301   aatgtcgacc cgaatgaatc cgtatttttt tctataaaaa tggaagaatt
351   ggtgtaattc gattctttcg tcaatgtgga atcgaatatt cattgatcaa
401   atgattcact ccatagtctg tagatctttt caagaactga ttaatcggac
451   gagaataaag atagagtccc gttctacatg tcaatgctgg caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt aaaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details