JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963268.1
Database:	nt	Go to original record
Description:	Melodinus cambodiensis chloroplast, partial genome.
Taxon:	Melodinus cambodiensis
Original size:	129002 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48112	48669	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acaaggatag gtgcagagac tcaacggaag ctgttctaac aaatggagtt
201   ggccgcgttg gtagagaaat ctttccatcg aaaattcaga aaggatgaag
251   gataaacgtt tatacatacg tattgaatac tatatcaaat gattaatgcc
301   gacccgaata aatccgtatt ttttctataa aaaatagaag aattggtgtg
351   attcgattcc acattgaaga aagaatcgaa tattcattga tcaaatgatt
401   cactccatag tccgtagatc ttttcaagaa ctgattaatc ggacgagaat
451   aaagatagag tcccgttcta catgtcaatg ctggcaacaa tgaaatttat
501   agtaagagga aaatccgtcg acttaaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details