JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963267.1
Database:	nt	Go to original record
Description:	Craspidospermum verticillatum chloroplast, partial genome.
Taxon:	Craspidospermum verticillatum
Original size:	128701 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48191	48766	+	576 bp

Amplicon:

576 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaggata aatgtatata catacgtatt gaatactata tcaaatgaaa
301   aatcaaatga ttaatgccga cccgaatgaa tctgtatttt ttctataaaa
351   aatggaagaa ttggtgtgat tcgattccac attgaagaaa gaatcgaata
401   ttcattgatc aaatgattca ctccatagtc tgtagatctt ttcaagaact
451   gattaatcgg acgagaataa agatagagtc ccgttctaca tgtcaatgct
501   ggcaacaatg aaatttatag taagaggaaa atccgtcgac ttaaaaaatc
551   gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	576	557	2.59e-08

Match details