JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963266.1
Database:	nt	Go to original record
Description:	Lepiniopsis trilocularis chloroplast, partial genome.
Taxon:	Lepiniopsis trilocularis
Original size:	129707 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47627	48200	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgatcacttt caaattcaga gaaaccctgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggggataggt gcagagactc aacggaagct gttctaacaa
201   atggagttgg ccgcgttggt agagaaattt ttccatcgaa aattcagaaa
251   ggatgaagga taaacgtata tacatacgta ttgaatacta tatcaaaatc
301   aaaggattaa tgccgacccg aatgaatctg tatttcttct ataaaaaaaa
351   tggaagaatt ggtgtgcttc gattccacat ggaagaaaga atcgaatatt
401   cattgatcaa atgattcact ccatagtctg tagatctttt cacgaaccga
451   ttaatcggac gagaataaag atagagtccc gttctacatg tcaatgctgg
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt aaaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details