JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963265.1
Database:	nt	Go to original record
Description:	Landolphia dawei chloroplast, partial genome.
Taxon:	Landolphia dawei
Original size:	129233 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48333	48895	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag cgataacttt caaattcaga gaaaccccgg aattaataaa
101   aggggcaatc ctgagccaaa tcccgttttc cacaagcaaa ggttcagaaa
151   acgaaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggccg cgttggtaga gaaatctttc catcgaaaat tcagaaagga
251   tgaaggataa acgtatatac atacgtattg aatactatat caaacgatta
301   atgccgaccc gaatgaatcc gtattttttc tataaaaaat ggaagaattg
351   gtctgattcg attccacatt gaagaaagaa tcgaatattc attgatcaaa
401   tgatttactc catagtctgt agatcttttc aagaactgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caatgctggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgactta aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details