JRC GMO-Amplicons | EURL GMFF

Record ID:	MG963264.1
Database:	nt	Go to original record
Description:	Lacmellea panamensis chloroplast, partial genome.
Taxon:	Lacmellea panamensis
Original size:	129768 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48522	49090	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aggggcaatc ctgagccaaa tcccgttttc cacaaacaaa ggttcagaaa
151   acgaaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggccg cattggtaga gaaatctttc catcgaaaat tcagaaagga
251   tgaaggataa acgtatatac atacgtattg aatactatat caaaatcaaa
301   cgattaatgc cgacccgaat gaatccgtat tttttctata aaaaatggaa
351   gaattggtct gattcgattc cacattgaag aaagaatcga atattcattg
401   atcaaatgat tcactccata gtctatagat cttttcaaga actgattaat
451   cggacgagaa taaagataga gtcccgttct acatgtcaat gctggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gacttaaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details